Intro To Plate Readers Microplate readers are also referred to as plate readers are laboratory instruments designed to detect biological, chemical or physical events in microplates¹ via the measurements of light. Plate readers are commonly found in many laboratories and are widely used for many applications across a wide range of disciplines, including life sciences, research, […]
Determination Of Membrane Potential
Application: Determination Of Membrane Potential Detection via fluorescence Black Pigmented Plates Black pigmented plates are optimized for fluorescence measurements. The plates show a minimal auto-fluorescence because of the black pigmentation, especially in the lower wavelengths, as well as minimized crosstalk between the wells. Black plates are available as standard, low volume or […]
Aequorin-Based Calcium Assay
Application: Aequorin-Based Calcium Assay Determination of intracellular calcium Detection of for calcium sensitive Aequorin Fluorescent Proteins Aequorin was originally obtained by the purification from the jellyfish (Aequorea victoria), but now it is prepared more efficiently using recombinant techniques. The active aequorin is formed by a complex between apoaequuorin (APO), oxygen and coelenterazine. On binding […]
Well Plate Colors
Selecting Well Plate Colors The application to be performed in the plate generally determines the well plate color. A basic rule to follow regarding the proper color section of microplates is listed below: Transparent Microplates Colorimetry or colorimetric assays are designed to detect or quantitate the amount of a particular reagent in an assay by […]