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Microplate Selection Information Source

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Aequorin-based Calcium AssayApplication: Aequorin-Based Calcium Assay

 

  • Determination of intracellular calcium
  • Detection of for calcium sensitive Aequorin

 

Fluorescent Proteins

Aequorin was originally obtained by the purification from the jellyfish (Aequorea victoria), but now it is prepared more efficiently using recombinant techniques. The active aequorin is formed by a complex between apoaequuorin (APO), oxygen and coelenterazine.  On binding calcium, the complex is broken emitting light and rendering the free apoaequorin and coeleteramide.

Active aequorin can be directly microinjected or obtained by reconstitution of apoaequorin with coelenterazine in transfected cells. In both cases, coelenterazine is needed to obtain the active complex (coelenterazine is burn out after light emission and is needed for reconstitution of aequorin). When using cells transfected with apoaequorin cDNA, the rate-limiting step in the reconstitution is the cell permeation of coelenterazine. Reconstitution of aequorin can be done using the native coelenterazine or analogs (some analogs with quicker response and more intese signal have been described and are comercially available). Although this protein was very popular for quantifying calcium in the past 30 years, it has been substituted by GFP derivatives. New derivatives combining aequorin and GFP or RFP (red fluorescence protein) have been developed based on the chemiluminescence resonance energy transfer process (CRET or BRET)¹.

White Pigmented Plates 

White pigmented plates are optimized for luminescence measurements. Because of the white coloration, the plates show a maximum reflection of the luminescence signals and minimize well-to-well crosstalk. White plates are available in standard or with transparent bottom. The white pigmented plates are available in the 96-, 384-, and 1536-well standard format and with transparent bottom. The microplates are offered in non-treated, immunological, and cell culture surfaces.

 

Application Criteria 

  • Surface Type: Non-Treated, Immunoassay, and Cell Culture
  • Cavities: 96-well, 384-well, and 1536-well
  • Material: Polystyrene
  • Well Plate Types:  Standard and Transparent Bottom
  • Color: White Pigmented Microplates
  • Bottom Types: F-Bottom, C-Bottom, and U-Bottom,

Product Selection Table

To help guide you through the microplate selection process we have developed a comprehensive selection table for white pigmented plates below. Products can be sorted by item number, surface type, number of cavities, microplate material, plate type, microplate color, bottom type and volume.

Questions? 

This is a comprehensive list of white pigmented microplates, if you have a specific question please feel free to contact us for more information.

Microplate Sample Program

We understand that selecting the right microplate is an important consideration and that is why we are excited to invite you to try our risk free sample program so that you can try the microplate in your laboratory before making your initial purchase.

Cat. No.SurfaceCavitiesMaterialTypeColorBottom typeVolume
781604pureGrade™96-wellPSStandardwhiteU330 µl
781605pureGrade™96-wellPSStandardwhiteF350 µl
781610pureGrade™96-wellPSTransp. BottomwhiteF330 µl
781621pureGrade™384-wellPSStandardwhiteF100 µl
781626pureGrade™384-wellPSTransp. BottomwhiteF120 µl
781641pureGrade™1536-wellPSStandardwhiteF10 µl
781665pureGrade™ S96-wellPSStandardwhiteF350 µl
781670pureGrade™ S96-wellPSTransp. BottomwhiteF330 µl
781681pureGrade™ S384-wellPSStandardwhiteF100 µl
781686pureGrade™ S384-wellPSTransp. BottomwhiteF120 µl
781701pureGrade™ S1536-wellPSStandardwhiteF10 µl
781724immunoGrade™96-wellPSStandardwhiteU330 µl
781726immunoGrade™96-wellPSStandardwhiteC350 µl
781741immunoGrade™384-wellPSStandardwhiteF100 µl
781784hydroGrade™96-wellPSStandardwhiteU330 µl
781786hydroGrade™96-wellPSStandardwhiteC350 µl
781801hydroGrade™384-wellPSStandardwhiteF100 µl
781844lipoGrade™96-wellPSStandardwhiteU330 µl
781846lipoGrade™96-wellPSStandardwhiteC350 µl
781850lipoGrade™96-wellPSTransp. BottomwhiteF330 µl
781852lipoGrade™96-wellPSTransp. BottomwhiteF330 µl
781861lipoGrade™384-wellPSStandardwhiteF100 µl
781866lipoGrade™384-wellPSTransp. BottomwhiteF120 µl
781868lipoGrade™384-wellPSTransp. BottomwhiteF120 µl
781904inertGrade™96-wellPSStandardwhiteU330 µl
781905inertGrade™96-wellPSStandardwhiteF350 µl
781906inertGrade™96-wellPSStandardwhiteC350 µl
781910inertGrade™96-wellPSTransp. BottomwhiteF330 µl
781912inertGrade™96-wellPSTransp. BottomwhiteF330 µl
781965cellGrade™96-wellPSStandardwhiteF350 µl
781970cellGrade™96-wellPSTransp. BottomwhiteF330 µl
781974cellGrade™96-wellPSTransp. BottomwhiteF330 µl
781981cellGrade™384-wellPSStandardwhiteF100 µl
781986cellGrade™384-wellPSTransp. BottomwhiteF120 µl
781988cellGrade™384-wellPSTransp. BottomwhiteF120 µl
782001cellGrade™1536-wellPSStandardwhiteF10 µl
782025cellGrade™ plus96-wellPSStandardwhiteF350 µl
782030cellGrade™ plus96-wellPSTransp. BottomwhiteF330 µl
782034cellGrade™ plus96-wellPSTransp. BottomwhiteF330 µl
782046cellGrade™ plus384-wellPSTransp. BottomwhiteF120 µl
782048cellGrade™ plus384-wellPSTransp. BottomwhiteF120 µl
782085cellGrade™ premium96-wellPSStandardwhiteF350 µl
782090cellGrade™ premium96-wellPSTransp. BottomwhiteF330 µl
782094cellGrade™ premium96-wellPSTransp. BottomwhiteF330 µl
782106cellGrade™ premium384-wellPSTransp. BottomwhiteF120 µl
782108cellGrade™ premium384-wellPSTransp. BottomwhiteF120 µl
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